Primer Premier

The Research of Applying Primer Premier 5.0 to Design PCR Primer

利用软件PrimerPremier5.0进行PCR引物设计的研究

The sequences of Enterobacter cloacae and staphylococcus epidermidis genes were designed using software Primer Premier 5.0 according to the literature .

我们根据文献报道，通过PrimerPremier5.0软件设计了实验所需的阴沟肠杆菌和表皮葡萄球菌的捕获探针，识别探针以及目标探针的DNA序列。

Using Primer Premier 5.0 software to design the microsatellite primers and finally obtained 266 microsatellite primer-pairs for the molecular genetics study of Laminaria .

使用PrimerPremier5.0软件对微卫星序列进行引物设计，最后设计得到266对微卫星引物可用于海带分子遗传学研究。

Both approaches have been tested in this study . Initially , specific PCR primers were designed with the Primer Premier 5.0 to amplify the conserved regions of human parvovirus B19 genome .

本研究首先利用PrimerPremier5.0软件针对细小病毒B19基因组保守区域设计PCR特异性引物。

Methods The probe and the primers of the assay were designed with the software packages of DNA Star and Primer Premier 5.0 . Their specificity and conservation were verified through Blast in GenBank and electronic hybridization .

方法用DNAStar和PrimerPremier5.0软件设计甲型流感病毒荧光PCR检测所用引物和探针；在GenBank中进行Blast以及电子对比证明其具有高度的特异性和保守性；

According to the reportes about highly conserved sequence of apple trees , pear trees or the other woody years plants and the model plant Arabidopsis thaliana , the proper primers were designed by primer premier 5.0 and oligo 6.0 software .

2利用PrimerPremier5.0、oligo6.0等软件根据已报道的苹果、梨等果树和模式植物拟南芥相关基因的高度保守序列设计了简并引物。

According to the sequence of RNA polymerase encoding region in genome of serotype ⅰ duck hepatitis virus ( DHV ⅰ), primers were designed with help of Primer Premier 5.0 design software , and a RT-PCR assay for the detection of DHV ⅰ was developed .

根据获得的Ⅰ型鸭病毒性肝炎病毒（DHVⅠ）RNA聚合酶基因序列，应用PrimerPremier5.0软件设计了1对引物，建立了检测DHVⅠ的RT-PCR方法。

Primer pairs and a probe were designed using software Primer Premier 5.0 . The specificity was validated through PCR detection of the saprophytic microorganism of citrus leaf surface , Tristeza virus and bacteria of Xac etc plant pathogens or G - / G + bacteria .

利用引物和探针设计软件PrimerPremier5.0进行引物和探针的设计；PCR同步扩增柑桔叶面腐生菌，柑桔溃疡病菌和衰退病菌等等植物细菌和病原物来验证检测的特异性；